An isophorone-derived AIE-active probe for peroxynitrite detection and bioimaging applications

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dc.contributor.authorLalitha, Raguraman
dc.contributor.authorVivek, Vishnu Halnor
dc.contributor.authorLu, Yueh-Hsun
dc.contributor.authorWu, Shu Pao
dc.contributor.authorVelmathi, Sivan
dc.date.accessioned2026-08-23T06:27:20Z
dc.date.available2026-08-23T06:27:20Z
dc.date.issued2025-07-11
dc.description.abstractCompounds exhibiting aggregation-induced emission (AIE) are remarkable due to their characteristic restriction of intramolecular rotation, making them highly versatile for a wide range of applications. Herein, an isophorone based probe (IHP) was developed for the detection of peroxynitrite (ONOO−) using the C 00000000 00000000 00000000 00000000 11111111 00000000 11111111 00000000 00000000 00000000 N as the reactive group. Photophysical investigations were conducted to explore the aggregation behaviour of IHP in two different solvent systems: DMSO:PBS and DMSO. In DMSO, only the monomeric form of the dye was observed, showing no unusual behaviour. However, in DMSO : PBS (1 : 1), multiple emitting species were detected, indicating the coexistence of dye aggregates and monomers. The fluorescence spectra exhibited wavelength-dependent changes, with the aggregates displaying a red-shift compared to the monomers, indicating J-aggregation. The AIE properties of IHP resulted in red emission, with a fluorescence response towards ONOO− through oxidative cleavage of the C N bond to an aldehyde accompanied by the shift in emission maximum. Under physiological conditions, the probe exhibited good selectivity and high sensitivity to ONOO−, low detection limit of 1.1 μM and rapid recognition within 200 seconds. Furthermore, the probe was effectively used to image exogenous and endogenous peroxynitrite in living cells. © 2025 The Royal Society of Chemistry.
dc.identifier.issn20462069
dc.identifier.urihttps://doi.org/10.1039/d5ra02185e
dc.identifier.urihttp://nitt.ndl.gov.in/handle/123456789/230
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.ispartofseriesRSC Advances; Vol. 15/ No. 30
dc.titleAn isophorone-derived AIE-active probe for peroxynitrite detection and bioimaging applications
dc.typeArticle
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