Browsing by Author "Lu, Yueh-Hsun"

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    An isophorone-derived AIE-active probe for peroxynitrite detection and bioimaging applications
    (Royal Society of Chemistry, 2025-07-11) Lalitha, Raguraman; Vivek, Vishnu Halnor; Lu, Yueh-Hsun; Wu, Shu Pao; Velmathi, Sivan
    Compounds exhibiting aggregation-induced emission (AIE) are remarkable due to their characteristic restriction of intramolecular rotation, making them highly versatile for a wide range of applications. Herein, an isophorone based probe (IHP) was developed for the detection of peroxynitrite (ONOO−) using the C 00000000 00000000 00000000 00000000 11111111 00000000 11111111 00000000 00000000 00000000 N as the reactive group. Photophysical investigations were conducted to explore the aggregation behaviour of IHP in two different solvent systems: DMSO:PBS and DMSO. In DMSO, only the monomeric form of the dye was observed, showing no unusual behaviour. However, in DMSO : PBS (1 : 1), multiple emitting species were detected, indicating the coexistence of dye aggregates and monomers. The fluorescence spectra exhibited wavelength-dependent changes, with the aggregates displaying a red-shift compared to the monomers, indicating J-aggregation. The AIE properties of IHP resulted in red emission, with a fluorescence response towards ONOO− through oxidative cleavage of the C N bond to an aldehyde accompanied by the shift in emission maximum. Under physiological conditions, the probe exhibited good selectivity and high sensitivity to ONOO−, low detection limit of 1.1 μM and rapid recognition within 200 seconds. Furthermore, the probe was effectively used to image exogenous and endogenous peroxynitrite in living cells. © 2025 The Royal Society of Chemistry.