Browsing by Author "Echeverria, Cesar"

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    Effect of morphology and (Sn, Cr) doping on in vitro antiproliferation properties of hydrothermally synthesized 1D GaOOH nanostructures
    (Elsevier B.V., 2024-03-15) Pilliadugula, Rekha; Haribabu, Jebiti; Mohamed Subarkhan, Mohamed Kasim; Echeverria, Cesar; Karvembu, Ramasamy; Gopalakrishnan, N
    GaOOH powders of submicron dimensions were synthesized through a simple, surfactant-free hydrothermal synthesis at pH values of 7 and 14. Sn and Cr at concentrations of 2 mol% and 1 mol%, respectively, were successfully incorporated into a GaOOH lattice at pH = 7. The synthesized powders were characterized through XRD, SEM, UV-Vis spectroscopy, XPS, and PL spectroscopy to untangle their structural, morphological, optical, elemental, and compositional- and defect-level analyses, respectively. All samples were individually tested for their in vitro anti-proliferation activities against three human cancer cell line profiles, namely, lung carcinoma (A549), colon adenocarcinoma (LoVo), and hepatocellular carcinoma (HuH-7). The samples showed obtrusive anti-proliferation activity against the three cancer line profiles. An apoptotic cell killing mechanism was found in the studied samples after a successful analysis of various staining assays and flowcytometric analyses in the A549 cancer cell profile. Cr loading at 1 mol% enhanced the cancer cell inhibition property of GaOOH against all cancer cells investigated. Also, the morphology and doping levels were observed to have a collective effect on the anti-proliferation activity of GaOOH with specificity in treating certain cancer cells. © 2021 The Authors
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    Host-guest interactions of coumarin-based 1,2-pyrazole using analytical and computational methods: Paper strip-based detection, live cell imaging, logic gates and keypad lock applications
    (Elsevier Ltd, 2024-01-05) Rasin, Puthiyavalappil; Basheer, Sabeel M; Haribabu, Jebiti; Aneesrahman, K.N.; Vadakkedathu Palakkeezhillam, Vishnunarayanan Namboothiri; Bhuvanesh, Nattamai; Echeverria, Cesar; Santibanez, Juan F; Sreekanth, Anandaram
    A novel Coumarin-based 1,2-pyrazole, HCPyTSC is synthesised and characterized. The chemosensor has been shown to have efficient colourimetric and fluorescence sensing capabilities for the quick and selective detection of fluoride and copper ions. At 376 and 430 nm, the HCPyTSC exhibits selective sensing for Cu2+ and F− ions. By examining the natural bond orbital (NBO) analysis and the potential energy curve (PES) of the ground state for the function of the C–H bond, it has been determined from the theoretical study at hand that the deprotonation was taken from the ‘CH’ proton of the pyrazole ring. For F− and Cu2+, the HCPyTSC detection limits were 4.62 nM and 15.36 nM, respectively. Similarly, the binding constants (Kb) for F− and Cu2+ ions in acetonitrile medium were found to be 2.06 × 105 M−1 and 1.88 × 105 M−1. Chemosensor HCPyTSC with and without F− and Cu2+ ions have an emission and absorption response that can imitate a variety of logic gates, including the AND, XOR, and OR gates. Additionally, a paper-based sensor strip with the HCPyTSC was created for use in practical, flexible F− sensing applications. The paper-based sensor was more effective in detecting F− than other anions. The effectiveness of HCPyTSC for the selective detection of F− in living cells as well as its cell permeability were examined using live-cell imaging in T24 cells. © 2024
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    Spectroscopic, anticancer and antioxidant studies of fluxional trans-[PdCl2(S-acylthiourea)2] complexes
    (Elsevier B.V., 2021-07-19) Dorairaj, Dorothy Priyanka; Haribabu, Jebiti; Chithravel, Vadivalagan; Vennila, Kailasam N; Bhuvanesh, Nattamai; Echeverria, Cesar; Hsu, Sodio C.N.; Karvembu, Ramasamy
    A library of palladium(II) acylthiourea complexes (1–6) of the type [PdCl2{C8H13N2OS(R)}2] [where R = C6H4CH3(o) (1), C6H5 (2), C6H4OCH3(p) (3), C6H4OC2H5(p) (4), C10H7 (5) or C6H5Cl(p) (6)] was synthesized and characterized by UV–Visible, FT-IR, 1H NMR, 13C NMR and ESI-Mass spectroscopy, and elemental analysis. Single crystal X-ray structure of complex 4 revealed the monodentate coordination of acylthiourea ligand through sulphur atom to the palladium ion in a trans fashion. In addition, variable-temperature (VT) NMR studies were performed to analyze the fluxional nature of complexes. The interaction of complexes with calf thymus (CT) DNA and BSA (bovine serum albumin) was analyzed by spectroscopic and molecular docking studies. The results inferred intercalation binding mode of the complexes with DNA. All the complexes exhibited good binding with BSA as well. Further, the complexes were found to act as good scavengers of DPPH as deduced from the antioxidant assay. In vitro cytotoxicity of the compounds against A549 (lung) cancer and HEK293 (human embryonic kidney) normal cell lines was investigated by MTT assay. Among the six complexes, complex 5 bearing a napthyl substitution in acylthiourea exhibited a remarkable activity against A549 cell line with an IC50 value of 14.8 µM and was more active than cisplatin (IC50 = 17.8 µM). On the other hand, it showed less toxicity on HEK293 cell line. The cell death mechanism was analyzed by AO/EB and DAPI staining, flow cytometry and Western blot investigations, which revealed that complex 5 could initiate cell death through apoptosis.